d allose Search Results


d allose  (MedChemExpress)
92
MedChemExpress d allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β d allose  (Thermo Fisher)
90
Thermo Fisher β d allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
β D Allose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d allose  (BOC Sciences)
93
BOC Sciences d allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d allose  (Biosynth Carbosynth)
93
Biosynth Carbosynth d allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d allose  (Chem Impex International)
95
Chem Impex International d allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+allose/D-Allose/pmc05408343__oc7b00085_si_001-30-0-4
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d-allose  (Carl Roth GmbH)
90
Carl Roth GmbH d-allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d-allose  (Omicron Biochemicals Inc)
90
Omicron Biochemicals Inc d-allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by Omicron Biochemicals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+allose/d+allose/pmc02597415-65-21-17
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d -allose  (FUJIFILM)
90
FUJIFILM d -allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d-allose  (Molekula GmbH)
90
Molekula GmbH d-allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
D Allose, supplied by Molekula GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β d allose  (Fluka Chemical)
86
Fluka Chemical β d allose
Dysregulation of the <t>Lm</t> <t>D-allose</t> operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.
β D Allose, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3-acetamido-3-deoxy-d-allose  (Biosynth Carbosynth)
N/A
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thymidine-5'-diphosphate-6-deoxy-d-allose  (Biosynth Carbosynth)
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Image Search Results


Dysregulation of the Lm D-allose operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.

Journal: mBio

Article Title: RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection

doi: 10.1128/mbio.01332-24

Figure Lengend Snippet: Dysregulation of the Lm D-allose operon leads to decreased virulence in mice. ( A ) Schematic of the ALO locus in Lm and expected expression under indicated conditions. ( B ) Relative gene expression of lmo0735 and lmo0737 in Lm grown to mid-log phase in BHI or BHI supplemented with 1% D-allose. Data are normalized to BHI for each gene. * P < 0.05 by Mann-Whitney analysis. ( C ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT, ∆ alsR , or ∆ alsR::alsR Lm ( n = 4/group) for 72 h. ( D ) CFU harvested from livers of WT mice infected via IV with 1 × 10 5 CFU of WT or ∆ alsR Lm for 4 ( n = 6/group) or 25 h ( n = 5/group). ** P < 0.01 by Mann-Whitney analysis. ( E ) Relative gene expression of the ALO in WT, ∆ alsR , and ∆ alsR::alsR Lm grown in BHI until mid-log phase. Data are plotted as fold change over WT for each gene. * P < 0.05, ** P < 0.01 by one way analysis of variance of ∆∆C t values. ( F ) WT mice were infected via IV with 1 × 10 5 CFU of WT ( n = 6), ∆ alsR ( n = 5), or ∆ alsR ∆alsOP ( n = 6) Lm. CFU were harvested from livers at 72 hpi. ( G ) WT mice were infected via IV with 1 × 10 5 CFU of the indicated strains ( n = 3 for ∆ alsR ∆ lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and ∆ alsR CFU were calculated from at least four independent experiments. CFU for individual gene mutants in ∆ alsR background were collected on independent days. Experiments in D, F, and G were performed once. CFU in C and F were analyzed by Kruskal-Wallis test with * P < 0.05.

Article Snippet: For ALO gene expression, overnight Lm were diluted to OD 600 = 0.05 in BHI or BHI supplemented with 1% D-allose (MedChem Express #HY-128741) and grown at 37°C with shaking until OD 600 = 0.4–0.6 and RNA was extracted as previously described ( ).

Techniques: Expressing, Gene Expression, MANN-WHITNEY, Infection